The switch‐liker's guide to plant synthetic gene circuits
JPB Lloyd, A Khan and R Lister
This review examines the core principles and components of plant synthetic gene circuits, including sensors, integrators, and actuators.
Publications
Deciphering seed development and germination in the single-cell era
P Schouveiler, LC Liew, M Corso, L Rajjou, J Zinsmeister, MG Lewsey
In this review, we discuss the current status of the application of single-cell technologies to seeds and present a workflow for conducting single-cell transcriptomics. Additionally, we discuss the integration of single-cell multi-omics.
Synthetic gene circuits in plants: recent advances and challenges
A Khan and R Lister
In this review, we discuss recent advances and challenges for synthetic gene circuits in plants.
MADS31 supports female germline development by repressing the post-fertilization programme in cereal ovules
X Yang, G Li, J Shi, LG Wilkinson, MK Aubert, K Houston, NJ Shirley, H Gao, RA Lister, L Colombo and MR Tucker
This study reveals that a B-sister MADS-box transcription factor, MADS31, is expressed in the inner subdomain of the nucellus and sustains germline development by preventing expression of post-fertilization genes.
Light‐activated channelrhodopsins: a revolutionary toolkit for the remote control of plant signalling
R Hedrich, M Gilliham
In this Tansley Insight, we provide an overview of the recent progress in the application of CHR optogenetics in higher plants and discuss their possible uses in the remote control of plant biology.
Light‐activated channelrhodopsins: a revolutionary toolkit for the remote control of plant signalling
R Hedrich and M Gilliham
In this Tansley Insight, we provide an overview of the recent progress in the application of CHR optogenetics in higher plants and discuss their possible uses in the remote control of plant biology.
Redefining the role of sodium exclusion within salt tolerance
S Garcia-Darga, SJ Roy, M Gilliham
This review discusses literature which shows that leaf Na+ accumulation can, in some circumstances, be tolerated without a detrimental effect on yield when HKT1 still functions to exclude Na+ from reproductive tissues.
Exploring consumer acceptability of leafy greens in earth and space immersive environments using biometrics
CG Viejo, N Harris, E Tongson & S Fuentes
This study used univariate and multivariate analysis on data from pick-and-eat leafy greens to assess self-reported and biometric consumer sensory analysis in simulated microgravity using reclining chairs and space-immersive environments.
Supply Chain Sustainability in Outer Space: Lessons to Be Learnt from Remote Sites on Earth
MV Hoyos, V Hessel, E Salas, J Culton, K Robertson, A Laybourn, M Escribà-Gelonch, N Cook & M de Zwart
This review describes learnings for human habitation in space from remote communities on Earth that have developed and survived over generations.
Optimising plant form and function for controlled environment agriculture in space and on earth
M Kamran, L Auroux, K Johnson & MG Lewsey
This review proposes several areas of research and development to produce plants suited to space settlements.
Green horizons: how plant synthetic biology can enable space exploration and drive on Earth sustainability
MF Morgan, M Gilliham & JC Mortimer
This review article explores how plant synthetic biology can enable space exploration and drive on Earth sustainability.
Conceptualising sustainability in outer space resource utilisation
M de Zwart, S Henderson & M Neumann
This article examines the meaning of the concept of sustainability with respect to space activities, with specific emphasis on the extraction and utilisation of space resources.
CRISPRi-based circuits to control gene expression in plants
MA Khan, G Herring, JY Zhu, M Oliva, E Fourie, B Johnston, Z Zhang, J Potter, L Pineda, J Pflueger, T Swain, C Pflueger, JPB Lloyd, D Secco, I Small, BN Kidd & R Lister
The construction of synthetic gene circuits in plants has been limited by a lack of orthogonal and modular parts. Here, we implement a CRISPRi-based reversible gene circuit platform in plants.
Digital Twins in Agriculture: Orchestration and Applications
M Escribà-Gelonch, S Liang, P van Schalkwyk, I Fisk, N Van Duc Long & V Hessel
In this review, Digital Twin technology is critically reviewed and framed in the context of recent advances in precision agriculture and Agriculture 4.0.
Assessment of changes in sensory perception, biometrics and emotional response for space exploration by simulating microgravity positions
CG Viejo, N Harris & S Fuentes
This study employed sensory trials and biometrics from video using simulated microgravity chairs to simulate alterations in sensory perception akin to those encountered in space conditions.